The data result of click chemistry tools is ideal

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Brand manufacturer: photosphotosolutions Original import article No.: 597-FTO Photosphotosolutions agent| The data result of click chemistry tools is ideal Product description: Product No.: 597-FTO Antibody name: FTO (Fat mass and antibody related protein) antibody Target: FTO (Fat mass and objective related protein) Scope of application: WB, IF Host: Mouse Quantity: 1 Antigen source: human Adaptive species: human, mouse, rat Clonicity: Monoclonal Storage condition: - 20 ℃ Form: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol Subtype: IgG The immunity: FTO (Fat mass and objectivity related protein) Specification: 100 μ L Photosphotosolutions agent| The data result of click chemistry tools is ideal 1. After receiving antibodies, please be sure to centrifuge at 12000 rpm for 1-5 minutes before opening the tube cover for sub packaging and storage! High quality antibodies use very special storage tubes. Only after centrifugation at 12000 rpm for 1-5 minutes can all antibodies attached to the tube wall or cover be centrifuged. Even centrifugation at 10000 rpm will lead to incomplete centrifugation, resulting in insufficient antibody! 2. For most antibodies, the appropriate storage method is to store them at - 20 ° or - 80 ° after subpackaging. For most antibodies, it is sufficient to store them at - 20 °. There is no evidence that saving at - 80 degrees will have more benefits! Note: There is also a note that it needs to be stored for 4 degrees. Do not freeze it! (1) The subpackaging can greatly reduce the damage of repeated freezing and thawing to the antibody activity, and also reduce the possibility of contamination caused by multiple absorption of antibodies from the same tube. (2) The amount of subpackage should be used up in one experiment, and the amount should not be less than 10ul per serving. Because the smaller the subpackage volume, the more likely the antibody concentration will be affected by evaporation and tube wall adsorption (3) If the sub packaged antibody after re melting cannot be used up at one time, the remaining mother liquor shall be stored at 4 ℃ to avoid re freezing! (4) Antibody working solution should be used up on the day of preparation, and should not exceed 1 day at 4 degrees. The data result of click chemistry tools is ideal (5) Excellent Avoid storing antibodies in the automatic defrosting refrigerator. Try to store the antibody in the refrigerator, not on the door. 3. After most antibodies are received, 4 degrees of temporary storage for 1-2 weeks has no effect on antibody activity. If the antibody will be used soon (1-2 weeks), it is recommended to store it at 4 degrees to avoid damage to antibody activity caused by repeated freezing and thawing. If long-term storage is required, the temperature should be - 20 ° or - 80 °* The key point is to store antibodies correctly according to the method recommended in the instructions! However, please freeze the ascites product immediately after receiving it! Because this kind of product contains a lot of protease, long-term storage at 4 degrees will lead to the degradation of antibodies! 4. Transportation conditions of most antibodies: The general transportation process takes 1-2 weeks, so it is completed under the condition of 4 degrees. The main purpose of 4-degree transportation * is to avoid the damage to antibody activity caused by repeated freezing and thawing (if dry ice is used for transportation, the antibody is frozen when it is received, and it needs to be unfrozen for subpackaging. This is an additional freezing and thawing process). Therefore, dry ice transportation shall be avoided during transportation! Storage conditions for special antibodies: The data result of click chemistry tools is ideal 1. Enzyme linked antibody: always keep it at 4 degrees to avoid freezing. Otherwise, the enzyme activity will be reduced or lost 2. Coupled antibodies: All the coupled antibodies are stored in a brown tube or in a tin foil away from light. In particular, fluorescent antibodies are extremely sensitive to light, so they should be operated in a dark place at all experimental stages! 3. Homotypic control of IgG3: always keep it at 4 degrees to avoid freezing. Because if freezing and thawing process occurs, the antibody is very easy to form a polymer Whether for storage or transportation, ！ Repeated freezing and thawing will lead to denaturation of antibody and formation of polymer, thus reducing the binding capacity of antibody