High performance liquid chromatography (HPLC) is a kind of chromatographic analysis technology, which depends on the different adsorbability of the packing materials in the chromatographic column to each component in the sample after pump pressurization, so as to realize the rapid separation of each component.
HPLC can realize the separation and identification of most substances.The birth of liquid chromatography stems from the limitations of gas chromatography in the analysis of high boiling organic substances, such as proteins, nucleic acids and other macromolecular substances that are not easy to gasify.Since the 1980s, with the introduction of chromatographic columns suitable for separating proteins and other biological macromolecules into the market, HPLC has developed its application in biochemistry.HPLC is the most widely used analytical technology at present. Mass spectrometry, as a separation technology, can compete with HPLC and can be used in many complementary ways, such asLC-MS。Dixintai detection platform uses the method of high performance liquid chromatography, which can identify samples and conduct quantitative analysis efficiently and accurately.
HPLC separation principle: the stationary phase is a solid adsorbent, the adsorbent is a porous particulate matter, and there is an adsorption center on the surface.The sample components compete with the mobile phase for the adsorption center.The different adsorption capacity of each component makes the retention time of the component in the stationary phase different, thus realizing separation.
Common detectors for high performance liquid chromatography
Characteristics of High Performance Liquid Chromatography
Wide application range:More than 70% of organic compounds can be analyzed by high performance liquid chromatography, especially those with high boiling point, macromolecule, strong polarity and poor thermal stability; High separation efficiency:The stationary phase and mobile phase can be selected to achieve the best separation effect, which is many times higher than the separation efficiency of industrial distillation tower and gas chromatography; Fast separation speed:Usually, the analysis of a sample can be completed within 15~30 minutes, and some samples can be completed within 5 minutes; High sensitivity:The sensitivity of uv detector can reach 0.01 ng; It can separate multiple substances at the same time:One column can separate up to 100 components at the same time; Small sample quantity and easy recovery:The injection amount is in the order of μ L, and the sample will not be damaged after passing through the chromatographic column, so a single component can be collected or prepared.
Detection service list
Measurable substance
cycle
Components of traditional Chinese medicine
2-3
Plant pigment
2-3
anthocyanin
2-3
Anthocyanin
2-3
Plant polyphenols
2-3
Plant flavonoids
2-3
alkaloid
2-3
Biogenic amine
2-3
Phytohormone
2-3
Animal hormone
2-3
Amino acids and their metabolites
2-3
vitamin
2-3
Organic acid
2-3
Sugar and sugar metabolites
2-3
Fats and fatty acids
2-3
Neurotransmitter
2-3
sterol
2-3
nucleotide
2-3
metallic element
2-3
food additives
2-3
Antibiotic residue
2-3
pesticide residue
2-3
Veterinary drug residue
2-3
Mycotoxin
2-3
Cyclosporin A/cyclosporin A/cyclosporin A
2-3
Lignan
2-3
Tween-20
2-3
glutaraldehyde
2-3
Other substances
Please contact technical support 182-0158-3820
Dixintai testing platform can customize other substance testing schemes according to needs. For details, please consult technical support 182-0158-3820 5-Fluorouracil sample test report,Click View> Test Report of Lignin Samples,Click View> Purity test/purity identification sample test report,Click View> Test report of zearalenone sample,Click View> Ochramycin A sample test report,Click View> Tween 20 sample test report,Click View>
