Determination of activity of some enzymes in insect glycolysis pathway, such as pyruvate dehydrogenase
This paper introduces how to use fluorescence imaging system to directly observe fluorescent proteins in Native PAGE without staining. At the same time, the common markers of Native PAGE, such as bovine serum albumin, blue native marker, cytochrome C, lactate dehydrogenase, etc., were introduced. These markers were used to verify the performance of the electrophoresis system, determine the electrophoresis conditions, and calibrate the molecular weight.