Qualified Hits Technologies
发现:
Inhibition- Whether allosteric,orthosteric or covalent Affinity- We measure KD,ligand efficiency and IC50。 Binding Kinetics- Essential binding kinetics such as Kon,Koff and residence time。 选择- Determine the KD as well as off target interaction analysis。 Binding mode- Use either X-ray crystallography or Cryo-EM to structurally determine the binding mode。
TheQualified Hits Engine
TheQualified Hits process
分析和规划
Team Assembly 目标access assment 目标分析 HTS library selection Screening cascade defined Assays identified 结构biology planning
平台支撑
生产效率 事故系统 生产成本
库扫描
火焰清理 [3khand-picked fragments] 范围查找记录 fragment enriched41ksmall molecule library Small molecule HTS [150k高质量small molecules] 报告装置 Enzymatic Assays SPR,MST,nanoDSF,nanoBRET,TSA
Hit Confirmation
报表分类分类分类分类 Binding affinity and kinetic profiling via SPR Enzymatic profiling of impact of inhibitors MicroScale Thermophoresis binding assays Label-free nanoDSF binding assays nanoBRET target engagement Luminescence interaction analysis using Alpha LISA Screening 交流声纳分析装置
Hit Characterization
报表分类分类分类分类 Enzymatic Assays Luminescence interaction analysis using Alpha LISA Screening Binding affinity and kinetic profiling via SPR MicroScale Thermophoresis binding assays Label-free nanoDSF binding assays 交流声纳分析装置 nanoBRET target engagement
结构装置
X-ray crystallogaphy Cryo-EM