Qualified Hits Technologies

Get qualified hits according your predetermined and fully customizable Research Target Profile(RTP)。

发现:
  • Inhibition-Whether allosteric,orthosteric or covalent
  • Affinity-We measure KD,ligand efficiency and IC50。
  • Binding Kinetics-Essential binding kinetics such as Kon,Koff and residence time。
  • 选择-Determine the KD as well as off target interaction analysis。
  • Binding mode-Use either X-ray crystallography or Cryo-EM to structurally determine the binding mode。

TheQualified Hits Engine

QualifiedHits-diagram QualifiedHits-diagram

TheQualified Hits process

分析和规划

  • Team Assembly
  • 目标access assment
  • 目标分析
  • HTS library selection
  • Screening cascade defined
  • Assays identified
  • 结构biology planning

平台支撑

  • 生产效率
  • 事故系统
  • 生产成本

库扫描

  • 火焰清理
    [3khand-picked fragments]
  • 范围查找记录
    fragment enriched41ksmall molecule library
  • Small molecule HTS
    [150k高质量small molecules]
  • 报告装置
  • Enzymatic Assays
  • SPR,MST,nanoDSF,nanoBRET,TSA

Hit Confirmation

  • 报表分类分类分类分类
  • Binding affinity and kinetic profiling via SPR
  • Enzymatic profiling of impact of inhibitors
  • MicroScale Thermophoresis binding assays
  • Label-free nanoDSF binding assays
  • nanoBRET target engagement
  • Luminescence interaction analysis using Alpha LISA Screening
  • 交流声纳分析装置

Hit Characterization

  • 报表分类分类分类分类
  • Enzymatic Assays
  • Luminescence interaction analysis using Alpha LISA Screening
  • Binding affinity and kinetic profiling via SPR
  • MicroScale Thermophoresis binding assays
  • Label-free nanoDSF binding assays
  • 交流声纳分析装置
  • nanoBRET target engagement

结构装置

  • X-ray crystallogaphy
  • Cryo-EM

Qualified Hits

在处理you will receive the deeply characterized qualified hits,

which meet the project specifications。