协议和时间

按最大似然比的Combining Molecular Profiling Methods

June 112024

1个

Dive into an in-depth exploration of the molecular methods for gene expression profiling—high lighting the strengths，applications，and uses of quantitative PCR（qPCR），Droplet Digital PCR（ddPCR），and RNA sequencing（RNA-Seq）technologies。

Articles 协议和时间表 Real-time qPCR/PCR

PCR Plates:5 Tips to Guide Your Buying Decision

August82023

304

Learn about5key aspects of a PCR plate that are crucial to your results.These tips provide you with the tools to make an informed decision when purchasing PCR plates。

Articles Break Room 电气/Western Blotting 协议和时间表

Resolved-Comic Series

January102202

9.7k

Follow along asanew starry-eyed graduate student learns the ins and outs of life asa research scientist，gaining valuable western blotting tips and tricks.Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator。

Articles Cell Biology Featured Stories 通用接口协议和时间表

6层到截面Funding for Flow Cytometers

June82021

1.6k

We asked core facilities staff and research scientists from the U.S.and Europe for their top tips for securing funding for large instrumentation，such as a cell analyzer or cell sorter.Here are the key questions you should consider while preparing your grant application to increase your chances of a successful outcome。

Articles Bio-Plex Multiplex Assays 协议和时间表 Research Highlights

Inflamed in the Brain:A Protocol for Studying Neuroinflammation in Mouse Brain Tissue

December8200

926

ice are critical model organisms for the study of neuroinflammation.But tissue quantity is often a limiting factor.In a recent paper，researchers describe a protocol for analysis of 33cytokines and chemokines using only50μl of tissue homogenate.The remaining homogenate can be used for for RNA extractionoralysis alysis。

Articles Cell Biology 协议和时间表

7 Tips for Purchasing Your Next Cell Sorter

September92020

2.2k

Presented here are some tips to help you avoid buyer’s remorse and make an educated decision about which instrument to purchase.These tips provide you with the tools to make an informed decision when purchasing a cell sorter。

Articles Cell Biology Featured Stories 协议和时间表

10 Tips为Setting Up a Successful Cell Sorting Experiment

June10220

7.9k

Incorporating fluorescence-based cell sorting intoyour lab grants you powerful access to new research areas and rarer cell types without sacrificing efficiency or results.New， powerful techniques like CRISPR and single-cell analysis are enhanced by the isolation of specific cells.But isolating cell populations of the right quality and quantity is both challenging and time consuming.We offer some tips to set yourself up for success and get the most out of your sorts。

Articles 协议和时间表 Real-time qPCR/PCR

工程用仪表板
CFX Real-Time PCR检测系统

November132019

4.2k

Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability.See how Bio-Rad’s family of CFX Real-Time PCR Detection Systems can measure protein thermal stability with higher throughput and more buffer systemsthan traditional circular dichroism detection。

Articles Featured Stories 协议和时间表 Real-time qPCR/PCR

Do More with Less:Five Steps to Great Results Using One-Step Multiplex RT-qPCR

September112019

4.9k

One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction.But proper optimization and validation is essential for its success.These five tips will have you designing primers and probes，selecting reporters，and validating and optiming your experimenta。

Articles 电气/Western Blotting 协议和时间表

10个焊缝故障诊断

February132019

4.1k

Being able to interrogate protein phosphorylation events and aberrant phosphorylation levels is critical to our understanding of various biological and pathological processes.However，detection of phosphorylated proteins in western blots can be challenging.These ten tips will help you generate robust data and clean blots。