| (NH4)2SO4 | 1.3 | g | | KH2PO4 | 0.28 | g | | MgSO4·7H2O | 0.25 | g | | CaCl2·2H2O | 0.07 | g | | FeCl3·6H2O | 0.02 | g | | MnCl2·4H2O | 1.8 | mg | | Na2B4O7·10H2O | 4.5 | mg | | ZnSO4·7H2O | 0.22 | mg | | CuCl2·2H2O | 0.05 | mg | | Na2MoO4·2H2O | 0.03 | mg | | VOSO4·nH2O | 0.03 | mg | | CoSO4·7H2O | 0.01 | mg | | Bacto Yeast Extract (Difco) | 1 | g | | Sulfur (powder) | 1 | g | | Distilled water | 1 | L | | pH 2.5 | | | | | Mix ingredients except Bacto Yeast Extract and sulfur, adjust pH to 2.5 with H2SO4, and autoclave at 121°C for 15 min. Separately autoclave Bacto Yeast Extract as a 10% solution. Sulfur is sterilized separately by autoclaving for 60 min at 105°C on each of 3 successive days. Prior to inoculation, add Bacto Yeast Extract solution and sulfur powder. Cultivate under air containing 1-10% CO2. |
|