| Hipolypepton* | 10 | g | | Yeast extract | 2 | g | | MgSO4·7H2O | 0.5 | g | | Na2CO3** | 10 | g | | Distilled water | 1 | L | | Agar (if needed) | 15 | g | | | | | | | | | | *Wako Pure Chemical Ind., Ltd., Osaka, Japan. | | | | | | | | | | **Sterilize separately by autoclaving and add to other ingredients. The final pH is between 9.0 and 10.0. |
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