| | (NH4)2SO4 | 1.3 | g | | | CaCl2·2H2O | 0.08 | g | | | MgSO4·7H2O | 0.25 | g | | | KH2PO4 | 0.28 | g | | | Na2SiO3·9H2O | 0.1 | g | | | Na2MoO4·2H2O | 0.03 | g | | | FeSO4·7H2O | 0.02 | g | | | Yeast extract* | 1 | g | | | Sulfur (powder)** | 10 | g | | | Distilled water | 1 | L | | | Adjust pH to 2.5 with 6 N H2SO4 | | | | | | | | | | | | | *Yeast extract is autoclaved separately as 10% solution. | | | | | | | | | | | | **Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days and added after inoculation. |
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