Proteome-wide Analysis in Saccharomyces cerevisiae Identifies Several PHD Fingers as Novel Direct and Selective Binding Modules of Histone H3 Methylated at Either Lysine 4 or Lysine 36

Xiaobing Shi; Ioulia Kachirskaia; Kay L. Walter; Jen-Hao A. Kuo; Aimee Lake; Foteini Davrazou; Steve M. Chan; David G. E. Martin; Ian M. Fingerman; Scott D. Briggs; LeAnn Howe; Paul J. Utz; Tatiana G. Kutateladze; Alexey A. Lugovskoy; Mark T. Bedford; Or Gozani

doi:10.1074/jbc.C600286200

图4

H3K36me3通过酿酒酵母PHD手指

A类，Ecm5的结构模型_（博士）与H3（1-6）K4me3络合物(右侧面板)和H3（33–38）K36me3(左侧面板)肽，如图3B，除了黄色的残留物表示Glu¹²⁵⁴（见“结果和讨论”）。B，Nto1的结构模型_（博士），表示形成芳香笼的残留物。残留物Phe²⁸⁵如所示黄色的（见“结果和讨论”）。C，使用所述GST-融合PHD突变体进行肽下拉分析图2B.